Differential expression of candidate defense genes of poplars in response to aphid feeding.
Identifieur interne : 003661 ( Main/Exploration ); précédent : 003660; suivant : 003662Differential expression of candidate defense genes of poplars in response to aphid feeding.
Auteurs : Claudio C. Ramírez [Chili] ; Fernando P. Guerra ; Rafael E. Zu Iga ; Cecilia CorderoSource :
- Journal of economic entomology [ 0022-0493 ] ; 2009.
Descripteurs français
- KwdFr :
- Amorces ADN (génétique), Analyse de variance (MeSH), Animaux (MeSH), Aphides (physiologie), Catechol oxidase (génétique), Catechol oxidase (métabolisme), Chitinase (génétique), Chitinase (métabolisme), Inhibiteurs de protéases (métabolisme), Interactions hôte-parasite (MeSH), Populus (génétique), Populus (parasitologie), RT-PCR (MeSH), Régulation de l'expression des gènes végétaux (génétique).
- MESH :
- génétique : Amorces ADN, Catechol oxidase, Chitinase, Populus, Régulation de l'expression des gènes végétaux.
- métabolisme : Catechol oxidase, Chitinase, Inhibiteurs de protéases.
- parasitologie : Populus.
- physiologie : Aphides.
- Analyse de variance, Animaux, Interactions hôte-parasite, RT-PCR.
English descriptors
- KwdEn :
- Analysis of Variance (MeSH), Animals (MeSH), Aphids (physiology), Catechol Oxidase (genetics), Catechol Oxidase (metabolism), Chitinases (genetics), Chitinases (metabolism), DNA Primers (genetics), Gene Expression Regulation, Plant (genetics), Host-Parasite Interactions (MeSH), Populus (genetics), Populus (parasitology), Protease Inhibitors (metabolism), Reverse Transcriptase Polymerase Chain Reaction (MeSH).
- MESH :
- chemical , genetics : Catechol Oxidase, Chitinases, DNA Primers.
- chemical , metabolism : Catechol Oxidase, Chitinases, Protease Inhibitors.
- genetics : Gene Expression Regulation, Plant, Populus.
- parasitology : Populus.
- physiology : Aphids.
- Analysis of Variance, Animals, Host-Parasite Interactions, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
Species belonging to the genus Populus (poplars) produce a series of defensive proteins in response to insect damage. Proteinase inhibitors, polyphenol oxidases, and chitinases are the most relevant and intensively studied proteins. Most of the knowledge about the relation between these proteins and herbivores has been obtained from studies with chewing insects. Nothing is known about whether phloem-feeder insects such as aphids are able to trigger a comparable response. In the current study, the expression of genes encoding a Kunitz trypsin inhibitor 3 (KTI3), a polyphenol oxidase 1 (PPO1), and a class I chitinase (CHI) was characterized in two poplar hybrids (one resistant hybrid and one susceptible hybrid, to aphids) attacked by the aphid Chaitophorus leucomelas Koch. The expression pattern was analyzed using a semiquantitative reverse transcription-polymerase chain reaction approach. The expression of KTI3 was increased by aphids only in the aphid-susceptible hybrid. Differently, PPO1 expression was increased by aphids in the aphid-resistant hybrid. The expression of CHI was down-regulated by aphids in the susceptible hybrid. This is the first study to report the differential expression of poplar defense genes in response to phloem-feeder insects such as aphids. The findings from the current study suggest that the expression levels of defensive proteins are affected by poplar genotype and by aphid infestation.
DOI: 10.1603/029.102.0327
PubMed: 19610421
Affiliations:
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Zu Iga</name></author><author><name sortKey="Cordero, Cecilia" sort="Cordero, Cecilia" uniqKey="Cordero C" first="Cecilia" last="Cordero">Cecilia Cordero</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2009">2009</date><idno type="RBID">pubmed:19610421</idno><idno type="pmid">19610421</idno><idno type="doi">10.1603/029.102.0327</idno><idno type="wicri:Area/Main/Corpus">003511</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">003511</idno><idno type="wicri:Area/Main/Curation">003511</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">003511</idno><idno type="wicri:Area/Main/Exploration">003511</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Differential expression of candidate defense genes of poplars in response to aphid feeding.</title><author><name sortKey="Ramirez, Claudio C" sort="Ramirez, Claudio C" uniqKey="Ramirez C" first="Claudio C" last="Ramírez">Claudio C. Ramírez</name><affiliation wicri:level="1"><nlm:affiliation>Instituto de Biología y Biotecnología Vegetal, Universidad de Talca, 2 Norte 685, Talca, Chile. clramirez@utalca.cl</nlm:affiliation><country xml:lang="fr">Chili</country><wicri:regionArea>Instituto de Biología y Biotecnología Vegetal, Universidad de Talca, 2 Norte 685, Talca</wicri:regionArea><wicri:noRegion>Talca</wicri:noRegion></affiliation></author><author><name sortKey="Guerra, Fernando P" sort="Guerra, Fernando P" uniqKey="Guerra F" first="Fernando P" last="Guerra">Fernando P. Guerra</name></author><author><name sortKey="Zu Iga, Rafael E" sort="Zu Iga, Rafael E" uniqKey="Zu Iga R" first="Rafael E" last="Zu Iga">Rafael E. Zu Iga</name></author><author><name sortKey="Cordero, Cecilia" sort="Cordero, Cecilia" uniqKey="Cordero C" first="Cecilia" last="Cordero">Cecilia Cordero</name></author></analytic><series><title level="j">Journal of economic entomology</title><idno type="ISSN">0022-0493</idno><imprint><date when="2009" type="published">2009</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Analysis of Variance (MeSH)</term><term>Animals (MeSH)</term><term>Aphids (physiology)</term><term>Catechol Oxidase (genetics)</term><term>Catechol Oxidase (metabolism)</term><term>Chitinases (genetics)</term><term>Chitinases (metabolism)</term><term>DNA Primers (genetics)</term><term>Gene Expression Regulation, Plant (genetics)</term><term>Host-Parasite Interactions (MeSH)</term><term>Populus (genetics)</term><term>Populus (parasitology)</term><term>Protease Inhibitors (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN (génétique)</term><term>Analyse de variance (MeSH)</term><term>Animaux (MeSH)</term><term>Aphides (physiologie)</term><term>Catechol oxidase (génétique)</term><term>Catechol oxidase (métabolisme)</term><term>Chitinase (génétique)</term><term>Chitinase (métabolisme)</term><term>Inhibiteurs de protéases (métabolisme)</term><term>Interactions hôte-parasite (MeSH)</term><term>Populus (génétique)</term><term>Populus (parasitologie)</term><term>RT-PCR (MeSH)</term><term>Régulation de l'expression des gènes végétaux (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Catechol Oxidase</term><term>Chitinases</term><term>DNA Primers</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Catechol Oxidase</term><term>Chitinases</term><term>Protease Inhibitors</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Gene Expression Regulation, Plant</term><term>Populus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Amorces ADN</term><term>Catechol oxidase</term><term>Chitinase</term><term>Populus</term><term>Régulation de l'expression des gènes végétaux</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Catechol oxidase</term><term>Chitinase</term><term>Inhibiteurs de protéases</term></keywords><keywords scheme="MESH" qualifier="parasitologie" xml:lang="fr"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="parasitology" xml:lang="en"><term>Populus</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Aphides</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Aphids</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Analysis of Variance</term><term>Animals</term><term>Host-Parasite Interactions</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de variance</term><term>Animaux</term><term>Interactions hôte-parasite</term><term>RT-PCR</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Species belonging to the genus Populus (poplars) produce a series of defensive proteins in response to insect damage. Proteinase inhibitors, polyphenol oxidases, and chitinases are the most relevant and intensively studied proteins. Most of the knowledge about the relation between these proteins and herbivores has been obtained from studies with chewing insects. Nothing is known about whether phloem-feeder insects such as aphids are able to trigger a comparable response. In the current study, the expression of genes encoding a Kunitz trypsin inhibitor 3 (KTI3), a polyphenol oxidase 1 (PPO1), and a class I chitinase (CHI) was characterized in two poplar hybrids (one resistant hybrid and one susceptible hybrid, to aphids) attacked by the aphid Chaitophorus leucomelas Koch. The expression pattern was analyzed using a semiquantitative reverse transcription-polymerase chain reaction approach. The expression of KTI3 was increased by aphids only in the aphid-susceptible hybrid. Differently, PPO1 expression was increased by aphids in the aphid-resistant hybrid. The expression of CHI was down-regulated by aphids in the susceptible hybrid. This is the first study to report the differential expression of poplar defense genes in response to phloem-feeder insects such as aphids. The findings from the current study suggest that the expression levels of defensive proteins are affected by poplar genotype and by aphid infestation.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">19610421</PMID><DateCompleted><Year>2009</Year><Month>08</Month><Day>31</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>13</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-0493</ISSN><JournalIssue CitedMedium="Print"><Volume>102</Volume><Issue>3</Issue><PubDate><Year>2009</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Journal of economic entomology</Title><ISOAbbreviation>J Econ Entomol</ISOAbbreviation></Journal><ArticleTitle>Differential expression of candidate defense genes of poplars in response to aphid feeding.</ArticleTitle><Pagination><MedlinePgn>1070-4</MedlinePgn></Pagination><Abstract><AbstractText>Species belonging to the genus Populus (poplars) produce a series of defensive proteins in response to insect damage. Proteinase inhibitors, polyphenol oxidases, and chitinases are the most relevant and intensively studied proteins. Most of the knowledge about the relation between these proteins and herbivores has been obtained from studies with chewing insects. Nothing is known about whether phloem-feeder insects such as aphids are able to trigger a comparable response. In the current study, the expression of genes encoding a Kunitz trypsin inhibitor 3 (KTI3), a polyphenol oxidase 1 (PPO1), and a class I chitinase (CHI) was characterized in two poplar hybrids (one resistant hybrid and one susceptible hybrid, to aphids) attacked by the aphid Chaitophorus leucomelas Koch. The expression pattern was analyzed using a semiquantitative reverse transcription-polymerase chain reaction approach. The expression of KTI3 was increased by aphids only in the aphid-susceptible hybrid. Differently, PPO1 expression was increased by aphids in the aphid-resistant hybrid. The expression of CHI was down-regulated by aphids in the susceptible hybrid. This is the first study to report the differential expression of poplar defense genes in response to phloem-feeder insects such as aphids. The findings from the current study suggest that the expression levels of defensive proteins are affected by poplar genotype and by aphid infestation.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ramírez</LastName><ForeName>Claudio C</ForeName><Initials>CC</Initials><AffiliationInfo><Affiliation>Instituto de Biología y Biotecnología Vegetal, Universidad de Talca, 2 Norte 685, Talca, Chile. clramirez@utalca.cl</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Guerra</LastName><ForeName>Fernando P</ForeName><Initials>FP</Initials></Author><Author ValidYN="Y"><LastName>Zuñiga</LastName><ForeName>Rafael E</ForeName><Initials>RE</Initials></Author><Author ValidYN="Y"><LastName>Cordero</LastName><ForeName>Cecilia</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D003160">Comparative Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Econ Entomol</MedlineTA><NlmUniqueID>2985127R</NlmUniqueID><ISSNLinking>0022-0493</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011480">Protease Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 1.10.3.1</RegistryNumber><NameOfSubstance UI="D004156">Catechol Oxidase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.14</RegistryNumber><NameOfSubstance UI="D002688">Chitinases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000704" MajorTopicYN="N">Analysis of Variance</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001042" MajorTopicYN="N">Aphids</DescriptorName><QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004156" MajorTopicYN="N">Catechol Oxidase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002688" MajorTopicYN="N">Chitinases</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="N">Gene Expression Regulation, Plant</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006790" MajorTopicYN="N">Host-Parasite Interactions</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000469" MajorTopicYN="Y">parasitology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011480" MajorTopicYN="N">Protease Inhibitors</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="entrez"><Year>2009</Year><Month>7</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2009</Year><Month>7</Month><Day>21</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>9</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">19610421</ArticleId><ArticleId IdType="doi">10.1603/029.102.0327</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Chili</li></country></list><tree><noCountry><name sortKey="Cordero, Cecilia" sort="Cordero, Cecilia" uniqKey="Cordero C" first="Cecilia" last="Cordero">Cecilia Cordero</name><name sortKey="Guerra, Fernando P" sort="Guerra, Fernando P" uniqKey="Guerra F" first="Fernando P" last="Guerra">Fernando P. Guerra</name><name sortKey="Zu Iga, Rafael E" sort="Zu Iga, Rafael E" uniqKey="Zu Iga R" first="Rafael E" last="Zu Iga">Rafael E. Zu Iga</name></noCountry><country name="Chili"><noRegion><name sortKey="Ramirez, Claudio C" sort="Ramirez, Claudio C" uniqKey="Ramirez C" first="Claudio C" last="Ramírez">Claudio C. Ramírez</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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